Spectral cytometry has revolutionized flow cytometry by harnessing full emission spectra. This advancement enables higher multiplexing and has resulted in increased demand for novel fluorophores and conjugates for deeper cell analysis. Here, we introduce BioLegend's cutting-edge conjugate families: Spark PLUS and StarBright, designed to fill critical spectral gaps and/or provide alternatives with superior performance. We explore their unique characteristics and show improvements to an existing 39-color Immune checkpoint panel. The new conjugates can replace previously used conjugates maintaining performance. The superior dye features results in higher resolution for several markers, improving data quality. Thus, BioLegend’s innovative technology offers unparalleled capabilities in multiparameter flow cytometry analysis.
