Th137 - The High-Throughput CyTOF XT PRO Mass Cytometer Enables High-Dimensional Immune Profiling with High Sensitivity, Repeatability and Reproducibility

Abstract Text: Reproducible immune profiling is crucial for translational and clinical research and developing accurate prognoses and effective treatments. Reproducibly identifying low-expressing targets and rare functional populations is challenging with fluorescence flow cytometry due to spectral spillover, autofluorescence and unmixing errors, issues not present with mass cytometry. Further, multiple workflows in mass cytometry provide flexibility and minimize technical variation, including the abilities to freeze metal-tagged antibody cocktails, barcode samples and freeze stained samples for future acquisition.
To assess the consistency of mass cytometry staining and acquisition workflows, human whole blood and PBMC were stained with antibody panels containing up to 50 surface and cytoplasmic targets. Samples were frozen and acquired using CyTOF™ XT PRO and CyTOF XT systems on a later date. The CyTOF XT PRO system allows for up to 4x increased acquisition rate compared with the CyTOF XT system. Both systems were highly repeatable and reproducible across multiple instruments. Cell population frequencies and signal intensities were not significantly different between the two CyTOF systems. Both systems had high sensitivity and dynamic range for abundant and low-abundance markers to accurately identify T helper cell populations.
Overall, these studies demonstrate that CyTOF XT and CyTOF XT PRO systems generate highly repeatable and reproducible data, and the 4x increased event rate of the CyTOF XT PRO system does not compromise data quality. Automated acquisition by CyTOF systems enables researchers to accurately and reproducibly streamline immunophenotyping and functional profiling, leading to accelerated biological insights.
For Research Use Only. Not for use in diagnostic procedures.